spectroscopic studies of the interaction of lysozyme and zno nanoparticles

Authors
abstract

abstract adsorption of proteins on inorganic surfaces may lead to structural and functional changes that are dependent on both the nature of the adsorbed proteins and the physicochemical properties of the inorganic surfaces. chicken egg white lysozyme (e.c 3.2.1.17, mw=14.6 kda) is a small globular protein, that consists of 129 amino acid residues with four disulfide bonds. the aim of this study was the survey of the stability and structure of chicken egg white lysozyme against zno nano through thermal stability, fluorescence and spectroscopy and enzyme activity assay in the absence or presence of zno nano particle at ph 7.0. the obtained results indicated that thermal stability and activity of lysozyme decreased with increase in zno nanoparticles concentration. moreover, it was observed that zno nano particle quenched the intrinsic fluorescence of lysozyme. the interaction studies of zno nanoparticles and lysozyme show that not only water and solvent molecules can effect on 3d structure of lysozyme and protein but also play an important role in adsorption nanoparticles.

Upgrade to premium to download articles

Sign up to access the full text

Already have an account?login

similar resources

Spectroscopic Studies on the Interaction of Nano-TiO2 with Lysozyme

In the present study, the interaction between nano-TiO2 and lysozyme was investigated by the method of UV-Vis detection and fluorescence spectroscopic techniques. The thermal denaturation of lysozyme has been investigated in the presence and absence of nano-TiO2 over the temperature range (293-373) K in different buffer and pH, using temperature scanning spectrosc...

full text

spectroscopic studies on the interaction of nano-tio2 with lysozyme

in the present study, the interaction between nano-tio2 and lysozyme was investigated by the method of uv-vis detection and fluorescence spectroscopic techniques. the thermal denaturation of lysozyme has been investigated in the presence and absence of nano-tio2 over the temperature range (293-373) k in different buffer and ph, using temperature scanning spectroscopy. the presence of nano-tio2 ...

full text

control of the optical properties of nanoparticles by laser fields

در این پایان نامه، درهمتنیدگی بین یک سیستم نقطه کوانتومی دوگانه(مولکول نقطه کوانتومی) و میدان مورد مطالعه قرار گرفته است. از آنتروپی ون نیومن به عنوان ابزاری برای بررسی درهمتنیدگی بین اتم و میدان استفاده شده و تاثیر پارامترهای مختلف، نظیر تونل زنی(که توسط تغییر ولتاژ ایجاد می شود)، شدت میدان و نسبت دو گسیل خودبخودی بر رفتار درجه درهمتنیدگی سیستم بررسی شده اشت.با تغییر هر یک از این پارامترها، در...

15 صفحه اول

comparative dna interaction studies of antiviral drug, zidovudine and its complex using different instrumental methods

هدف از این مطالعه بررسی امکان استفاده از داروهای شناخته شده در درمان سایر بیماریها به عنوان داروهای ضد سرطان است. همچنین با استفاده از این داروها در ساختمان کمپلکس فلز می توان شاخص های دارویی بدست آمده را بررسی نمود. داروی ضد ویروس ایدز(hiv)به نام زیدوودین(azt)انتخاب و.کمپلکس.محلول.در.آب[pt(azt)2]cl2سنتزو به روشهای مختلف فیزیکی و شیمیایی شناسایی گردید. بر هم کنش مقایسه ای این دارو و کمپلکس پلا...

15 صفحه اول

postnatal studies of bats (pipistrellus kuhlii and miniopterus schreibersii) & histomorphology and histochemistry studies of organs and diseases of (neurergus microspilotus and n. kaiseri)

1. to determine whether difference in birth body mass influenced growth performance in pipistrellus kuhlii we studied a total of 12 captive-born neonates. bats were assigned to two body mass groups: light birth body mass (lbw: 0.89 ± 0.05, n=8) and heavy birth body mass (hbw: 1.35 ± 0.08, n=4). heavier body mass at birth was associated with rapid postnatal growth (body mass and forearm length) ...

Structural studies on the interaction of nano-SiO2 with lysozyme

The interaction between nano-SiO2 and lysozyme was investigated by the method of UV-Visible  detection and fluorescence spectroscopic techniques. The thermal denaturation of lysozyme has been investigated in the presence and absence of nano-SiO2 over the temperature range (293-373) K in different buffers and pH values, using temperature scanning spectroscopy. The presence of nano-SiO2 caused th...

full text

My Resources

Save resource for easier access later


Journal title:
زیست شناسی جانوری تجربی

جلد ۴، شماره ۴، صفحات ۱-۹

Keywords

Hosted on Doprax cloud platform doprax.com

copyright © 2015-2023